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Effects of lpriflavone on bone remodeling in the rat calvarial cell
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KMID : 0361920050350040275
Abstract
º» ¿¬±¸´Â ipriflavone (isopropoxyisoflavone)ÀÇ Åõ¿©°¡ ¹é¼ µÎ°³°ü¼¼Æ÷ÀÇ Áõ½Ä°ú °ñÁ¶Á÷ °³Á¶¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ¾Ë¾Æº¸°íÀÚ ½ÃµµµÇ¾ú´Ù. ÅÂ·É 20-21ÀÏ°ÀÇ ¹é¼ µÎ°³°ü¼¼Æ÷¸¦ ºÐ¸® ¹è¾çÇÑ ÈÄ, 10^(-9) MºÎÅÍ 10^(-5) M±îÁö ³óµµÀÇ ipriflavoneÀ» Åõ¿©ÇÏ°í 1ÀÏ°¿Í 3ÀÏ°¿¡ MTT ºÐ¼®À» ½ÃÇàÇÏ¿© Èí±¤µµ¸¦ Æò°¡ÇÑ °á°ú, ¸ðµç ³óµµ¿¡¼ ¹é¼ µÎ°³°ü¼¼Æ÷ÀÇ Áõ½ÄÀ» º¸ÀÌÁö ¾Ê¾Ò´Ù. ÇÑÆí °ñÁ¶Á÷ °³Á¶¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ¾Ë¾Æº¸±â À§ÇÏ¿© 14ÀÏ°¿¡ alizarin red¿°»öÀ» ½ÃÇàÇÏ¿©, Çü¼ºµÈ ¼®È¸È °áÀý ¸éÀûÀ» ÃøÁ¤ÇÏ¿´À» ¶§, 10^(-8) M, 10^(-7) M, 10^(-6) M ³óµµ¸¦ Åõ¿©ÇÑ °æ¿ì ¼®È¸È °áÀý Çü¼ºÀÌ À¯ÀÇÇÏ°Ô Áõ°¡ÇÏ¿´´Ù. °ñ¾Æ¼¼Æ÷ÀÇ ºÐÈ¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ¾Ë¾Æº¸±â À§ÇÏ¿© ipriflavoneÀ» Åõ¿©ÇÏ°í 7ÀÏ°¿Í 14ÀÏ°¿¡ ÃßÃâÇÑ RNA¸¦ ¿ªÀü»ç ÁßÇÕÈ¿¼Ò ¿¬¼â¹ÝÀÀ(RT-PCR)À» ½ÃÄÑ bone sialoprotein (BSP), type 1 collagen (COL I), osteocalcin (OCN) À¯ÀüÀÚ ¹ßÇöÀ» °üÂûÇÑ °á°ú BSP¿Í COL I À¯ÀüÀÚ´Â ¹è¾ç 7ÀÏ° ³ôÀº ¹ßÇöÀ» º¸¿´°í, OCN À¯ÀüÀÚ´Â ¹è¾ç 14ÀÏ° ³ôÀº ¹ßÇöÀ» º¸¿´´Ù. ÀÌ»óÀÇ ¿¬±¸°á°ú ipriflavoneÀÌ ¹é¼ µÎ°³°ü¼¼Æ÷¿¡¼ ¼®È¸È¸¦ ÃËÁø½ÃÅ°°í °ñ¾Æ¼¼Æ÷ÀÇ ºÐÈ¿¡ °ü¿©ÇÏ´Â BSP, COL I ¹× OCN À¯ÀüÀÚ ¹ßÇöÀ» Áõ°¡½ÃÄÑ °ñÁ¶Á÷ÀÇ °³Á¶¸¦ ºü¸£°Ô ÇÒ ¼ö ÀÖÀ½À» ½Ã»çÇÏ¿´´Ù.
lpriflavone (isoprofoxyisoflavone), a synthetic derivative from soy isoflavone diazein, has been shown to inhibit bone resorption and perhaps stimulate bone formation. This study was performed to examine the effects of ipriflavone on the proliferation and bone remodeling in rat calvarial cells in vitro. The rat calvarial cells were isolated from fetus aged 20 to 21 days and cultured in BGJb media. The graded concentration of ipriflavone (10^(-9)-10^(-5) M) was administered into cultured cells. When the cell proliferation was estimated through the measurement of MTT assay, there was no increase in cellular proliferation of the rat calvarial cell at any ipriflavone concentration. The cellular activity was evaluated through the formation of mineralized nodules stained by alizarin red. The formation of mineralized nodules significantly increased at concentrations of 10^(-8) M, 10^(-7) M and 10^(-6) M ipriflavone. Reverse transcription-polymerase chain reaction analyses (RT-PCR) were done at 7 and 14 days after culture to detect the expression of Bone Sialoprotein (BSP), Type I collagen (COL I) and Osteocalcin (OCN). As a result, the expressions of BSP and COL I increased of the 7th day of culture and the expression of OCN increased on the 14th day of culture. These results indicate that ipriflavone facilitates the bone remodeling process by promoting rat calvarial cell differentiation and stimulating mineralization through increased expression of extracellular matrix genes, such as BSP, COL I and OCN.
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Ipriflavone;µÎ°³°ü¼¼Æ÷;°ñÁ¶Á÷ °³Á¶;Calvarial cell;Bone remodeling
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